FastQCFastQC Report
Tue 7 May 2019
SRR8168089.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8168089.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35088864
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG45950.031.408483
TTACGAC51700.028.462845
TCGTGCG41000.026.1436427
CGATCAC108500.025.4766123
GTTACGA61100.024.5452654
TACGACT69500.021.3750676
TAGCATA113350.021.2775758
ACGACTT81400.019.4634887
GTGCGTC54900.019.1829059
AGGCGTA60050.018.711618
CCACGAT111450.018.3171671
CACGATC197950.017.4892431
CTCGATT121400.015.83317548-49
ACGATCA178250.015.7199912
GCCAGTA155200.015.5691713
GCGATTT135300.015.1964894-95
TGCGCGA89000.014.7913119
TACGCAC121100.014.57157750-51
TCGCATT134450.014.0922332
ATTCCGT137350.014.04748252-53
Produced by FastQC (version 0.11.2)