FastQCFastQC Report
Tue 7 May 2019
SRR8168090.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8168090.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34839177
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT385420.11062833085867671No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT371780.10671319819064613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC143850.026.246093
ACGATCA143350.026.1440682
CACGATC171100.023.1790771
CCACGAT138750.021.0899721
TCACAAG268800.014.7980256
ATTGGAC285200.014.2603186
CTCGAAT91350.013.7505881
TATGCCG60350.012.23154453
GAATTGG374400.012.1818934
CTCGCAT82150.012.0262521
CTGGAAT588050.011.8883921
AACGCGT60100.011.73483
TCGCATT63200.011.6814072
AATTGGA369500.011.6438055
TAATGTA281250.011.5521924
CTCCCGT110450.011.5004981
ATCACAA370600.011.190555
AAGACGT292650.011.15174614-15
CACAAGA376800.010.9934957
GGAATTG438050.010.9372833
Produced by FastQC (version 0.11.2)