FastQCFastQC Report
Tue 7 May 2019
SRR8168091.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8168091.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33228503
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT362210.10900581347284889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG50650.035.9341663
TTACGAC54350.034.7880445
GTTACGA61950.030.6717324
TCGTGCG44000.028.7533257
TACGACT76050.025.1076536
TAGCATA125100.024.9640438
ACGACTT86600.022.5924787
GTGCGTC59200.021.213079
TCTAACG52050.020.0614912
GCCAGTA155050.020.04963
AGGCGTA66700.019.7440558
TGCGCGA95500.017.5331769
TCACCCG225650.017.090493
CTCGATT157000.016.8343148-49
CCAGTAG192000.016.6083584
CTTGTTA121350.016.5973661
CACCCGG235400.016.0837654
GTAGCAT201150.015.7823717
ATTCCGT169150.015.76590652-53
GCGATTT175700.015.734158594-95
Produced by FastQC (version 0.11.2)