FastQCFastQC Report
Tue 4 Dec 2018
SRR5517162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5517162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48663670
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG8407011.7275741841912047TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT3037080.624095963169239TruSeq Adapter, Index 3 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAACTCGTATG623620.12814898670815417TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA359850.041.0326651
TCGTATG1174700.039.3241944
TGCGCAA103500.034.68242636
ACTCGTA106800.033.40503342
GCGCAAC104600.032.97190537
ATCGGAA1410750.032.4948121
TAATGCG1435600.032.13363633
CGTCTGA1440800.032.1317615
ACGTCTG1445500.032.06659314
CTCGTAT1423750.032.06561743
CGGAAGA1459050.031.794333
AATGCGC1465350.031.6842834
ACTAATG1452950.031.68353831
ATGCGCA1461750.031.5377535
TCGGAAG1492950.031.412242
CTAATGC1478950.031.32880832
GTCACTA1478750.031.09764128
ATCTCGT1321650.030.7731441
GCGCATC1316750.030.72741937
CGCAACT113300.030.45971338
Produced by FastQC (version 0.11.2)