FastQCFastQC Report
Wed 24 Apr 2019
SRR5517149_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5517149_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47327442
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC13551392.8633261015881653Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT4963871.048835472662985Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)