FastQCFastQC Report
Wed 24 Apr 2019
SRR5517151_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5517151_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43165687
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT8655442.0051667427417526Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC4408911.021392292447471Illumina Single End PCR Primer 1 (96% over 31bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCTC4393361.0177898940887933Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCT1639900.3799082359096937Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCCATGTGTAGATCTC585540.13564941060708707Illumina Single End PCR Primer 1 (96% over 31bp)
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC553450.12821526505532044No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT526160.12189311385221323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)