FastQCFastQC Report
Wed 24 Apr 2019
SRR5517165_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5517165_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50200777
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC10809842.153321252378225Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCT10190442.029936707951751Illumina Single End PCR Primer 1 (96% over 32bp)
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT545040.10857202469196842No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC514980.10258406956529775No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)