FastQCFastQC Report
Sun 6 Aug 2017
G150-M2-Index-2_CGATGT-_BCALRAANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M2-Index-2_CGATGT-_BCALRAANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21387232
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG12250.017.24332812
GCGTCGT12700.017.15210511
TCGTGTA14450.015.22673914
AGCGTCG14700.014.66880310
GTCGTGT16050.013.84599413
GATCGGA15850.013.4637621
ATCGGAA16800.013.3569652
CGTGTAG18900.012.10727215
AGAGCGT23800.09.7072288
GAGCGTC23800.09.5223279
GATCTCG19150.09.41949134
TGGTCGC20000.09.130177542
ATCTCGG21900.08.5380235
AAGAGCG28100.08.2217237
GTGGTCG22150.08.14373241
TCGGAAG28950.08.1310953
CGGAAGA34800.06.9542554
TCGGTGG31150.06.567592638
TCTCGGT28450.06.495002736
GTAGATC32250.06.27566531
Produced by FastQC (version 0.11.2)