FastQCFastQC Report
Sun 6 Aug 2017
G150-M5-Index-5_ACAGTG-_BCALRAANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M5-Index-5_ACAGTG-_BCALRAANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20417999
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG16650.026.42008843
CTCGTAT17050.025.54401242
TCTCGTA16450.025.4056141
CGTATGC17550.025.31648644
ATCGGAA21150.021.7362292
GATCGGA21150.021.646491
CGTCTGA23750.019.07981916
ATCTCGT22000.018.99623540
GATCTCG22250.018.88239139
ACACGTC24700.018.61552613
ACGTCTG28600.015.84421615
TCGGAAG31350.014.8005683
CACGTCT34450.013.53753714
CGGAAGA35650.013.0775944
CACACGT41700.011.65950412
GCACACG50750.09.58027511
AGTGATC49150.08.99706136
GTGATCT53650.08.24211137
GAACTCC67300.07.456594521
TGATCTC64150.07.16645438
Produced by FastQC (version 0.11.2)