FastQCFastQC Report
Sun 6 Aug 2017
G150-M15-Index-15_ATGTCA-_BCALRAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M15-Index-15_ATGTCA-_BCALRAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24578938
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT13650.022.21108644
TCTCGTA13600.020.8369243
GATCGGA15450.019.6843871
ATCGGAA16900.018.633392
AATCTCG16750.016.78636641
CGTCTGA20500.015.03858716
ACACGTC20950.014.71550213
ATCTCGT19500.014.41888142
TCGGAAG24950.012.8859643
ACGTCTG24650.012.68460115
CGGAAGA29600.010.8624684
CACGTCT32700.09.831429514
GCACACG39050.08.571033511
CACACGT40650.08.12571912
ACATGTC69600.05.08277632