FastQCFastQC Report
Sun 6 Aug 2017
G150-M19-Index-20_GTGGCC-_BCALRAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG150-M19-Index-20_GTGGCC-_BCALRAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21657223
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG10900.029.6760912
GCGTCGT11000.029.40637811
AGCGTCG12000.026.95584310
GATCGGA15100.021.2718141
GTCGCCG12750.021.05384444
TCGTGTA16550.019.54466414
ATCGGAA18100.018.3538842
GTCGTGT18600.017.86396413
GAGCGTC19250.017.1463789
GATCTCG16750.017.07217834
TGGTCGC15850.016.93328342
CGTGTAG20850.015.724833515
GTGGTCG18450.014.90325241
GGTCGCC19150.014.70628343
ATCTCGG21250.013.97433935
AGAGCGT23700.013.9266548
AAGAGCG25850.012.9387067
TCTCGGT23450.012.66325336
TCGGAAG27200.012.052723
TCGGTGG26050.011.48374638
Produced by FastQC (version 0.11.2)