FastQCFastQC Report
Tue 12 Jul 2016
SRR072108_NA18507_ATAC_GENOMIC_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR072108_NA18507_ATAC_GENOMIC_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18378252
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN557770.303494586971601No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN392400.21351323292334873No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN281700.15327899519497284No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN221650.1206045058039252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGG1200.002956125410.00054210
TACGCCG1809.331128E-69.9941945
TATACCG2601.0011718E-89.8019985
TATACTG26600.09.7499765
TACACTG34700.09.6773765
TACAGGT72500.09.319784
TAGACTG28100.09.0694655
TAGACAG46650.09.0301245
TAATACT29500.08.8875724
TACACAG45050.08.8849615
GGCTAGA34950.08.7937121
GGTATAT29200.08.5743091
ATAGGAC16200.08.5087853
TAGGACT25850.08.4617354
CTGTCGC17250.08.3474689
TCTGTCG15650.08.337338
TCTATAC20500.08.3319333
GTAGGAC13950.08.270113
ACTAGAC23050.08.1901983
GGTATAC14650.08.1868711
Produced by FastQC (version 0.11.2)