FastQCFastQC Report
Tue 12 Jul 2016
SRR072109_NA18507_ATAC_GENOMIC_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR072109_NA18507_ATAC_GENOMIC_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18559340
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN571610.30799047810967417No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN406540.21904873772450958No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN283930.1529849660602155No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN230930.12442791607891229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG38300.010.2958855
TACACCG4550.010.2154385
TATACTG26800.09.6227765
GTCTACG4650.09.0298341
TACACAG44900.08.7490655
TTACACT33550.08.6630154
TAGACAG53050.08.648545
TACAGGT73200.08.6369674
TTATACT28800.08.6352624
GTAGGAC15450.08.6303053
TAATACT25250.08.4846624
TAGGACT29200.08.4656634
GGTCTAC13050.08.2736421
CTACACT28600.08.2241974
CTATACT22600.08.1537354
CTAACAC41550.08.1489573
TAACACT30750.08.136384
CCTACAC25600.08.1346623
TGAGCCG31700.08.0942910
CTTACAC27650.08.0733873
Produced by FastQC (version 0.11.2)