FastQCFastQC Report
Tue 12 Jul 2016
SRR072109_NA18507_ATAC_GENOMIC_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR072109_NA18507_ATAC_GENOMIC_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18559340
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1755620.9459495865693499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG49000.09.8728145
TACACTG34800.09.7951245
TATACTG25400.09.7281025
CTGTCGC18850.09.0076099
TAGACTG27300.08.9965295
TCTATAC17800.08.8680783
TGTCGCC18700.08.76762510
GTACTAG12350.08.68549251
GTGTAAG27450.08.5751881
TCTACAC25950.08.550523
TAATACT22650.08.5445544
TCTGTCG18350.08.5196568
CTAGACA44400.08.4830464
CTATACT22250.08.4305284
GTATTAG25100.08.309661
TACACCG4302.1827873E-118.3080015
GTGTTAG38400.08.3025491
CTAGGAC25750.08.2699423
TTATACT26500.08.2582054
GTATAGA21900.08.1633051
Produced by FastQC (version 0.11.2)