FastQCFastQC Report
Tue 12 Jul 2016
SRR891272_GM12878_ATAC_CHROMATIN_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR891272_GM12878_ATAC_CHROMATIN_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23259350
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA233500.10038973574068064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA36750.027.11606644
ATCTCGT41050.024.3828343
GACGGAC46350.022.0219632
ACGAGAC53200.019.22789828
TATCTCG52650.019.01073342
CCCACGA58850.017.53141225
TCCGAGC62050.017.23132319
CGAGACG59650.017.14877129
CGAACGA12150.016.83807814
CGAGCCC62200.016.62388621
AGACGGA64900.015.93102331
CCGAACG13250.015.60622313
CGACGTT8250.015.46535414
TCGGCGT8650.015.00450711
CCACGAG71000.014.624269526
ACGGACT70850.014.53175333
GCCCACG72000.014.35995524
CGTGCGA8400.014.14165810
CCGAGCC78200.013.92590720
CGGACTC74600.013.88973834
Produced by FastQC (version 0.11.2)