FastQCFastQC Report
Wed 13 Jul 2016
SRR891272_GM12878_ATAC_CHROMATIN_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR891272_GM12878_ATAC_CHROMATIN_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23259350
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG36650.026.85108643
CTCGGTG39950.025.0165942
CGGTGGT39500.024.52353144
CGACGAG40450.024.48029528
CCGACGA40750.024.29813827
GCCGACG42550.023.58036626
CGAGTGT42950.022.8014531
TGCCGAC44350.022.76937325
TGACGCT45950.022.30775319
GACGAGT45250.021.83453229
CTGCCGA48550.021.0710624
TCTCGGT50100.020.56284141
ACGCTGC50850.020.24652521
GTAGATC50500.020.22434836
ACGAGTG51150.019.18721230
GACGCTG56350.018.3092620
CGCTGCC59750.017.3048822
GATCTCG61750.016.96786739
GCTGCCG61250.016.73749223
TAGATCT65450.016.00861237
Produced by FastQC (version 0.11.2)