FastQCFastQC Report
Wed 13 Jul 2016
SRR891273_GM12878_ATAC_CHROMATIN_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR891273_GM12878_ATAC_CHROMATIN_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22898819
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGT377510.1648600305544142Illumina Single End PCR Primer 1 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG51900.034.2290743
CGGTGGT53600.032.36290444
CTCGGTG55350.032.17481242
CCGACGA58250.030.86011927
CGACGAG58250.030.82295228
GCCGACG60450.029.84665726
TGCCGAC61850.029.38406225
CGAGTGT61400.028.74164631
GACGAGT62000.028.6035929
TGACGCT64000.028.5266619
CTGCCGA65000.028.12919224
GTAGATC66500.027.07203336
TCTCGGT68200.027.04818341
ACGCTGC67400.026.89587221
CGCTGCC74300.024.63609122
ACGAGTG72150.024.58154330
GCTGCCG74950.024.39377623
GACGCTG77900.023.58045420
GATCTCG77550.023.50258339
TAGATCT77700.023.37263137
Produced by FastQC (version 0.11.2)