FastQCFastQC Report
Tue 12 Jul 2016
SRR891274_GM12878_ATAC_CHROMATIN_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR891274_GM12878_ATAC_CHROMATIN_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24184246
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA1487080.6148961600870253No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC341740.14130686563476075No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG256770.10617242315513992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA274600.024.34190444
ATCTCGT280200.023.89500643
TATCTCG319750.021.11836442
ATGCCGT58250.020.65455244
GACGGAC340050.020.3657932
TATGCCG68600.019.2696944
ACGAGAC374000.018.59582928
CCCACGA376800.018.5043825
AGACGGA375650.018.47859831
CGAGCCC379850.018.36601421
GAGACGG387150.018.01492130
TCCGAGC391200.017.97895619
CGTGCGA22050.017.85300410
GCCCACG392200.017.8049524
CGAGACG395200.017.73723429
CCCAACG26650.017.660821
CCGAGCC405450.017.46097820
CGGACTC404050.017.23313334
CGCAAAG27350.017.20772212
ACGGACT403700.017.20449333
Produced by FastQC (version 0.11.2)