FastQCFastQC Report
Wed 14 Jun 2017
SRR1177966_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177966_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18974404
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC263530.13888710285709108TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC77200.025.62444144-45
TATGCCG89550.022.17008846-47
CGATGTA102150.019.52844234-35
TCACCGA103350.019.4165930-31
TGCCGTC110450.017.97493248-49
ATCTCGT117200.016.73705940-41
TATCTCG85000.016.70740338-39
CCGATGT90650.016.0852332-33
CTCGTAT122700.015.90940742-43
CACCGAT94200.015.63030730-31
CACGTCT128050.015.58546814-15
ACCGATG129900.015.53947932-33
CACACGT129850.015.38270912-13
GATCGTA27650.014.6010835
ATCGGAA204400.014.3603262
GATCGGA209200.014.0989451
ATGTATC138400.014.07033736-37
CGTCTGA145850.014.01503816-17
CGGAAGA216850.013.9958124
GTCACCG108300.013.99003828-29
Produced by FastQC (version 0.11.2)