FastQCFastQC Report
Wed 14 Jun 2017
SRR1177966_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177966_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18974404
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA34100.015.60697846-47
GATCGTA29700.014.2272815
CGTATCA37400.013.14650850-51
GTCGAAC19450.012.6825521
GCCGTAT40100.012.203032548-49
ACTAGCC58600.011.585825
CGCGTAT6653.8307917E-911.4135641
GGATCGT42750.011.3259764
CGCCGAT14250.011.3184511
CGGAAAC67450.010.6901691
CGGGAAT34200.010.6804011
CCACTAG70700.010.6736963
CCGTATC54700.010.37948-49
ATCGTAC46050.010.2069546
CCGGGAT60450.010.1231651
CGATTAG26100.010.003084
CGCGAAT18550.09.973411
CTGAAAT186100.09.8138031
CTAGCCT76600.09.2893531
CCGCAAT22700.09.1949581
Produced by FastQC (version 0.11.2)