FastQCFastQC Report
Wed 14 Jun 2017
SRR1177969_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177969_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19310282
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCC74350.015.901865
CTAGCCT99200.015.8775471
CCACTAG82100.015.2075763
TAGCCTC116400.013.086512
GATCGTA30600.012.10327155
CACTAGC99950.011.9708154
CCCCACT143750.011.5179331
GTCGAAC22150.010.7091071
CCGGCAT45800.010.4619561
CGGGCAT48900.010.1867921
CTGAAAT185250.09.8852141
CTCGGAT60550.09.8721871
CGCGGTT22300.09.7861081
CGGCGAT20700.09.625781
CGCCGAT16300.09.604691
GGATCGT42200.09.5634024
CCGCAAT22300.09.3606241
CTCGATT34050.09.3350081
CTGGAAT206550.09.0955091
GGGATCG56000.09.0707493
Produced by FastQC (version 0.11.2)