FastQCFastQC Report
Wed 14 Jun 2017
SRR1177970_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177970_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19120119
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC356930.18667770843894851TruSeq Adapter, Index 2 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG202850.10609243593096883TruSeq Adapter, Index 2 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC102850.030.6884544-45
TATGCCG116600.027.25284446-47
CGATGTA126800.025.2853434-35
TCACCGA133800.024.47723830-31
TGCCGTC132600.024.19725448-49
ATCTCGT139650.022.6695840-41
CACGTCT152650.021.51959614-15
CACACGT154700.021.12593312-13
CTCGTAT157150.020.28114142-43
CGTCTGA167150.019.80984516-17
ATGTATC159350.019.71796236-37
ACCGATG172650.019.01059532-33
TATCTCG110450.018.16959638-39
GTATCTC184150.017.9910638-39
ATCGGAA233650.017.8093762
GATCGGA237150.017.5665651
CACCGAT120650.017.49962230-31
CGGAAGA246050.017.1617934
CCGATGT119800.017.0885332-33
TGCTTGA190400.016.47745558-59
Produced by FastQC (version 0.11.2)