FastQCFastQC Report
Wed 14 Jun 2017
SRR1177985_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177985_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16537337
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGC164150.050.905769
GAAGACG218500.038.8515747
AAGACGG226050.037.826998
GCATACG81150.036.3217514-15
GATCGGG47200.035.3755231
ATCGGGA50250.032.1963922
CGGCATA95400.031.7457312-13
CAAGCAG237350.030.6376291
GACGGCA117400.029.07685510-11
AGCAGAA293700.029.0540963
AAGCAGA322750.026.769032
CATACGA103350.026.36119714-15
GCAGAAG314900.026.108844
AGAAGAC306750.025.7240286
CGGCGTG52150.025.6234212-13
ATACGAG107000.025.23810216-17
AGAGCGG81800.024.4355078
CGAGATT79950.024.33657318-19
GGCATAC123800.024.2906412-13
GAGCGGC74950.023.8816819
Produced by FastQC (version 0.11.2)