FastQCFastQC Report
Wed 14 Jun 2017
SRR1177986_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177986_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15559784
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1722111.1067698626150595TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA391450.053.9849171
ATCGGAA394150.053.4343452
CGGAAGA407050.052.2658774
TCGGAAG412100.050.876363
AGAGCAC467350.045.4178968
GAGCACA493550.042.9876489
AAGAGCA523900.040.4874847
GAAGAGC543700.039.0777976
CGTATGC295750.035.58212744-45
TATGCCG305150.034.70395746-47
GGAAGAG629900.034.2499925
AATCTCG299100.033.27792438-39
TGCCGTC321400.033.0010548-49
ATCTCGT307500.032.5156140-41
TCGTATG319350.032.3948442-43
GACCAAT325400.032.33263834-35
ACCAATC321300.032.03561836-37
CTCGTAT316900.032.03075842-43
TCTCGTA305700.031.88356640-41
GTATGCC337100.030.84408844-45
Produced by FastQC (version 0.11.2)