FastQCFastQC Report
Wed 14 Jun 2017
SRR1177986_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177986_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15559784
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGC124600.039.517369
GCATACG54650.032.6039614-15
GAAGACG168700.028.8774937
AAGACGG178450.027.9116928
CGGCATA67550.026.7330412-13
GACGGCA85500.023.29539110-11
GATCGGG38600.023.2277831
CAAGCAG190750.021.9598561
CATACGA77850.021.45530314-15
ATCGGGA40850.020.9086912
ATACGAG79700.020.24136416-17
AGCAGAA244650.019.9669063
GGCATAC93700.019.72817412-13
GACGCGT13300.019.47682444-45
CGGCGTG41950.019.28907812-13
CGAGATT62000.019.01495218-19
AAGCAGA272800.018.315992
ACGAGAT68150.017.50784318-19
AGAAGAC257600.017.4738946
GCAGAAG269500.017.22914
Produced by FastQC (version 0.11.2)