FastQCFastQC Report
Wed 14 Jun 2017
SRR1178004_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178004_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19622049
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA40700.016.51088146-47
GTCGAAC17850.014.9181181
CGACAAT57950.014.852151
CGCGTAT5108.767529E-1013.9857341
GATCGTA26250.012.4847555
CGTCGTG61300.012.04831512-13
CCGTATC58500.011.64950748-49
GATCTCG68850.011.55878634-35
CCACTAG66150.011.3563653
CCGGCAT37500.011.2855551
CGTATCA39500.011.00108550-51
AGCGTCG71100.010.98733510-11
CTCGTAT51700.010.9451221
CGGGTAT18300.010.9134591
ACTAGCC61950.010.7336385
CGCGGCA14900.010.5315391
CGCGGCT24400.010.5236921
CTAGCCT77000.010.4366271
GCCGTAT42600.010.423548-49
GCGGAAT53000.010.2280581
Produced by FastQC (version 0.11.2)