FastQCFastQC Report
Wed 14 Jun 2017
SRR1178011_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178011_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20653804
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1034320.500789104031393TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA288550.039.777351
ATCGGAA293600.039.157652
CGGAAGA312850.036.674564
TCGGAAG327950.035.08733
AGAGCAC431700.026.9173248
CGTATGC239550.025.2931544-45
GAGCACA460550.025.2720959
TATGCCG250450.024.23056446-47
ACGATCA253900.023.46672432-33
AAGAGCA491800.023.43487
TCACGAT257300.023.25785830-31
AGTCACG259650.023.2192628-29
GAAGAGC500500.023.131816
TGCCGTC270350.022.30061148-49
CACACGT276650.022.07286612-13
ATCTCGT262700.021.8472940-41
GATCTCG246100.021.72553838-39
CACGTCT283750.021.62933214-15
CACGATC264650.021.1577330-31
ACACGTC269850.021.0981912-13
Produced by FastQC (version 0.11.2)