FastQCFastQC Report
Wed 14 Jun 2017
SRR1178011_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178011_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20653804
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATACG42950.025.87153614-15
GATCGGG44750.021.4490071
ATCGGGA45100.020.8660182
AGACGGC141800.019.2509089
CGGCATA62500.018.23189512-13
ACTAGCC76700.017.6521385
CGCCGTA68800.016.52958146-47
CCACTAG83900.016.256293
GACGGCA78400.015.31966710-11
CTAGCCT104500.015.00536251
TCGGGGG93550.014.97172938-39
TCGGGAG63950.014.4165943
CGGCGTG37950.014.13732612-13
AAGAGCG184100.013.9322927
GATCGGA203100.013.8503461
GAGCGGC75000.013.672639
ATCGGAA205250.013.5695482
CCGTATC89300.013.56023348-49
AGAGCGG76750.013.552588
CACTAGC105350.013.3932684
Produced by FastQC (version 0.11.2)