FastQCFastQC Report
Wed 14 Jun 2017
SRR1178012_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178012_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18300443
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC676450.36963586072752447TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA237800.034.6867941
ATCGGAA241350.034.255332
CGGAAGA256350.032.0471534
TCGGAAG271850.030.377143
CGTATGC191900.022.77708644-45
AGAGCAC374950.022.567768
GAGCACA389800.021.7078919
TATGCCG203600.021.55046546-47
ACGATCA202450.021.0904132-33
AGTCACG207700.020.87588928-29
TCACGAT207450.020.741530-31
ATCTCGT206300.019.90057440-41
AAGAGCA417250.019.8585937
CACACGT222600.019.84922412-13
GATCTCG194150.019.62198638-39
CACGTCT226650.019.49456614-15
GAAGAGC428450.019.483556
TGCCGTC224800.019.48247748-49
ACACGTC217000.019.03758412-13
CACGATC213450.018.8665430-31
Produced by FastQC (version 0.11.2)