FastQCFastQC Report
Wed 14 Jun 2017
SRR1178013_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178013_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16094612
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCC54150.015.9603225
CCACTAG62500.014.82296853
GTCGAAC11950.014.7229191
CTAGCCT72600.012.9684681
CCCCACT115300.012.0836571
CACTAGC77450.011.8338174
CGTATGC63400.011.7213544-45
CGACAAT31550.011.153021
GATCGGA114500.010.9222061
GATCGTA20100.010.867525
TAGCCTC85000.010.7315272
ATCGGAA117150.010.7063372
CGCGTAT4003.7375285E-410.6989861
CGGGCAT37350.010.56691
TATGCCG73700.010.341310546-47
CGCCGAT8209.116775E-99.8581441
CCGCAAT15950.09.8381471
CGGGAAT23950.09.7286071
CGGAAGA131800.09.6562464
CGGGTAT21200.09.6447891
Produced by FastQC (version 0.11.2)