FastQCFastQC Report
Wed 14 Jun 2017
SRR1178070_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178070_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46264487
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT220200.015.7550981
GTCGAAC37850.014.7957561
ACTAGCC157550.014.5035695
CCACTAG183150.013.1493133
TAGCCTC251200.013.0409132
CGACAAT74350.011.6812991
CACTAGC206550.011.544874
GATCGTA59250.011.2017525
CGCGTTT16950.010.9198321
CCCCACT327250.010.8188281
CTGAAAT479650.010.7257061
GATCGGA351200.010.5404981
CGATTAG54450.010.2737834
ATCGGAA367650.010.0837422
CGTATGC205400.010.02377444-45
CGGGAAT68800.09.8643541
CACGAAT239950.09.6125051
CCGGCAT94850.09.6069371
CGGAAGA397100.09.5746034
CGGAAAC206900.09.4735031
Produced by FastQC (version 0.11.2)