FastQCFastQC Report
Wed 14 Jun 2017
SRR1178070_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178070_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46264487
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT221600.015.7566451
GCATACG56150.015.43191814-15
ACTAGCC155250.014.8398025
TAGCCTC247900.013.5675962
CCACTAG183850.013.3215163
GATCGTA61000.012.7712085
GTCGAAC40700.012.2557831
CGGCGAC75050.011.9634731
CGACAAT73850.011.192961
CCCCACT336700.011.1462851
CACTAGC217300.010.9888414
CGCGTAT12350.010.77053451
CTCGGAT142750.010.7491121
CTCGATT74450.010.4646651
CTGAAAT478450.010.4354771
GGCGACC82350.09.9799582
CGGAAAC205250.09.8830481
CGGGAAT69450.09.7816011
CGGGCAT109300.09.7358541
CGACGAT41500.09.7300931
Produced by FastQC (version 0.11.2)