FastQCFastQC Report
Wed 9 May 2018
LZ_M10.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M10.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18537691
Sequences flagged as poor quality0
Sequence length33
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC4774282.5754448059361867Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGGATAATAGTATGCCATTCCCCATTAATCTT233310.12585709838404363No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTT223780.12071622080657186No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATA191400.10324910475635828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA508450.026.846171
ACGTCTG517850.026.34352715
CGGAAGA519800.026.3363574
CGTCTGA518200.026.31271416
ATCGGAA519950.026.1812532
GCACACG528300.025.86841611
ACACGTC529300.025.79404613
CACACGT531100.025.71170812
AGCACAC535200.025.61810710
TCGGAAG532900.025.5195733
CACGTCT536600.025.42302514
AGAGCAC540500.025.4118428
GAACTCC541900.025.14974821
GAGCACA548050.025.066699
AACTCCA547100.024.92158322
AAGAGCA555050.024.8234947
CAGTCAC533450.024.82098827
GTCTGAA550750.024.78717
TCCAGTC552100.024.6180725
ACTCCAG554350.024.58591323
Produced by FastQC (version 0.11.2)