FastQCFastQC Report
Wed 9 May 2018
LZ_M11.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M11.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15815089
Sequences flagged as poor quality0
Sequence length33
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC7488474.73501603437072Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGGATAATAGTATGCCATTCCCCATTAATCTT295540.18687216998905287No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATA241130.15246831680808118No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGA235690.14902856379752275No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAG218220.1379821510963359Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTT212940.13464356729197036No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAAT202670.12814976886946383No Hit
CAGAATATTCACCAGCCTTTTCTGTTTTGTCAG187850.11877897114584686No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGG182180.11519378740138611No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGG168990.10685365096585926No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA161540.10214295980250253No Hit
CCAGGATAATAGTATGCCATTCCCCATTAATCT159530.10087202164970428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA809450.026.9534651
ATCGGAA816300.026.6767712
CGGAAGA822350.026.6593864
ACGTCTG823350.026.52276415
CGTCTGA823100.026.51934216
GCACACG829050.026.36319511
TCGGAAG826200.026.3137633
ACACGTC830950.026.28830113
CACACGT832300.026.24403812
AGCACAC839400.026.09278710
CACGTCT837050.026.0838314
AGAGCAC841700.026.0776758
GAACTCC844800.025.85453221
GAGCACA848750.025.8466789
CAGTCAC817250.025.84303527
AAGAGCA850550.025.8206187
AACTCCA848400.025.72887822
ACTCCAG848800.025.7231123
GAAGAGC854300.025.6930586
GTCTGAA854450.025.60477317
Produced by FastQC (version 0.11.2)