FastQCFastQC Report
Sun 13 May 2018
LZ_M14.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M14.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21128626
Sequences flagged as poor quality0
Sequence length33
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC2426571.1484750593815236Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA259850.026.6720851
CGTCTGA277450.025.96146816
ACGTCTG278500.025.87819115
ATCGGAA273100.025.3677312
CGGAAGA283750.025.3136374
GCACACG286750.025.17715811
ACACGTC292250.024.65638513
CACACGT295500.024.41718312
AGCACAC300000.024.19070610
CACGTCT300350.024.03143514
AGAGCAC303300.023.9808548
TCGGAAG294450.023.6103323
GAACTCC306200.023.52376221
CAGTCAC302000.023.12249827
AACTCCA311850.023.08901622
GAGCACA315400.023.0566279
GTCTGAA315350.022.94833417
ACTCCAG317300.022.67967223
AAGAGCA323850.022.621717
TCCAGTC316950.022.5684325
Produced by FastQC (version 0.11.2)