FastQCFastQC Report
Sun 13 May 2018
LZ_M15.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M15.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19071290
Sequences flagged as poor quality0
Sequence length33
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC3711091.9459040264187686Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA407700.026.7665621
CGTCTGA427400.026.18310716
ACGTCTG428400.026.13781215
ATCGGAA420450.025.8876462
CGGAAGA432400.025.8831234
GCACACG438300.025.60956411
ACACGTC441000.025.42195913
CACACGT443850.025.26480912
AGCACAC446350.025.21396410
CACGTCT448750.024.98533814
TCGGAAG437050.024.9377083
AGAGCAC452200.024.908618
GAACTCC451100.024.79246521
GAGCACA458850.024.5623879
AACTCCA456550.024.4906622
GTCTGAA460750.024.33772717
CAGTCAC445550.024.32574827
CTGAACT462950.024.20425819
ACTCCAG462700.024.1301323
AAGAGCA469900.024.1226187
Produced by FastQC (version 0.11.2)