FastQCFastQC Report
Sun 13 May 2018
LZ_M16.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M16.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14893882
Sequences flagged as poor quality0
Sequence length33
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC2559571.7185378533279636Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA269700.026.706311
ACGTCTG275250.025.91417715
CGTCTGA275200.025.908916
ATCGGAA278000.025.8413072
CGGAAGA278750.025.688944
GCACACG282750.025.25561111
TCGGAAG289550.024.8007723
AGCACAC289250.024.7487410
GAACTCC291150.024.46204821
AGAGCAC293350.024.4382578
ACACGTC294750.024.22731213
AACTCCA294200.024.18164822
CACACGT296900.024.0518712
GAGCACA298750.024.025019
GTCTGAA297600.023.9995917
CTGAACT297150.023.97233619
ACTCCAG299800.023.69724323
CAGTCAC294150.023.61082527
CACGTCT302250.023.60811414
TCCAGTC300450.023.56959725
Produced by FastQC (version 0.11.2)