FastQCFastQC Report
Sun 13 May 2018
LZ_M17.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M17.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18628611
Sequences flagged as poor quality0
Sequence length33
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC4166672.2367046045462002Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA453650.026.7649481
CGTCTGA458050.026.21322616
ACGTCTG458300.026.20587215
ATCGGAA463000.026.1663532
CGGAAGA465850.026.0372374
GCACACG469050.025.68016411
AGCACAC477100.025.30628810
TCGGAAG479600.025.2795663
ACACGTC476400.025.26696413
GAACTCC478000.025.15609421
CACACGT479250.025.13360812
AGAGCAC484500.024.9905368
CACGTCT483700.024.87154614
GAGCACA490200.024.7126229
AACTCCA488550.024.62727222
CAGTCAC474700.024.5233227
GTCTGAA490600.024.5208217
AAGAGCA497000.024.4842197
TCCAGTC492550.024.28691525
ACTCCAG495900.024.24527223
Produced by FastQC (version 0.11.2)