FastQCFastQC Report
Sun 13 May 2018
LZ_M18.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M18.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17163512
Sequences flagged as poor quality0
Sequence length33
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC4549032.6504074457488653Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCATTGGATAGGTCAATGATATTTTCTCTAAGG311750.1816353203237193No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATA295730.1723015662528741No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTT278300.16214630199227292No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGA236590.1378447487903408No Hit
CCAGGATAATAGTATGCCATTCCCCATTAATCT220660.12856343154011837No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCC213550.12442092271092303No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAAT207840.1210940977580812No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTC203520.11857713036819038No Hit
GCCAGGATAATAGTATGCCATTCCCCATTAATC182260.10619038807442208No Hit
CAGAATATTCACCAGCCTTTTCTGTTTTGTCAG176930.10308496303087619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA491550.026.8357331
ACGTCTG494600.026.27247615
CGTCTGA495100.026.23896216
CGGAAGA501200.026.2044074
ATCGGAA504800.026.0743682
GCACACG503750.025.85943211
TCGGAAG509650.025.7964973
ACACGTC504700.025.77598613
CACACGT506450.025.70024912
AGCACAC510850.025.52909510
CACGTCT510650.025.48086214
GAACTCC512400.025.30616221
AGAGCAC517550.025.259298
GAGCACA520350.025.1200839
CAGTCAC501750.025.11989627
AACTCCA516900.025.1127722
AAGAGCA527650.024.901147
ACTCCAG522050.024.82064223
TCCAGTC522800.024.71811725
GAAGAGC537750.024.4835786
Produced by FastQC (version 0.11.2)