FastQCFastQC Report
Sun 13 May 2018
LZ_M19.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M19.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15591267
Sequences flagged as poor quality0
Sequence length33
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC2100481.3472157201848958Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA229600.026.5981671
ATCGGAA235350.025.8555742
CGTCTGA233700.025.79553216
ACGTCTG234200.025.75347515
CGGAAGA240450.025.3065224
GCACACG239500.025.30225611
ACACGTC245150.024.66931513
CACACGT247100.024.49102212
AGCACAC250900.024.2871210
CACGTCT251550.024.02534114
TCGGAAG253500.023.9985923
AGAGCAC254000.023.9729148
GAACTCC253500.023.79176521
GAGCACA259550.023.4621019
GTCTGAA259700.023.30136117
AACTCCA260250.023.27917122
CAGTCAC253900.023.1539627
AAGAGCA268800.022.8036297
CTGAACT265350.022.7950419
ACTCCAG265300.022.7898223
Produced by FastQC (version 0.11.2)