FastQCFastQC Report
Wed 9 May 2018
LZ_M1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20504618
Sequences flagged as poor quality0
Sequence length33
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC9592004.677970591795468Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGGATAATAGTATGCCATTCCCCATTAATCTT369740.18032035515121522No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGA312320.15231690734253134No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATA296890.14479177324834824No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCAG286510.13972949898408252Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTT285090.1390369720616107No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAAT259180.12640079420157938No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA244580.11928044697053122No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGG224040.1092631913454813No Hit
CAGAATATTCACCAGCCTTTTCTGTTTTGTCAG222890.1087023420772823No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGG220880.10772207509547362No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGA212870.10381563801871364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1035650.026.9476571
ATCGGAA1041750.026.700232
CGGAAGA1052200.026.6064554
ACGTCTG1053000.026.47613715
CGTCTGA1053450.026.46354516
ACACGTC1060100.026.2987513
GCACACG1056150.026.28077311
CACACGT1056950.026.25832712
TCGGAAG1059400.026.196163
AGCACAC1065600.026.1274910
CACGTCT1067800.026.09779714
AGAGCAC1068900.026.068458
GAGCACA1079700.025.820039
CAGTCAC1042100.025.78300727
AAGAGCA1084000.025.7750457
GAACTCC1081450.025.7710521
AACTCCA1086150.025.66475522
ACTCCAG1088650.025.5810223
GTCTGAA1091850.025.56002417
TCCAGTC1084000.025.55391525
Produced by FastQC (version 0.11.2)