FastQCFastQC Report
Sun 13 May 2018
LZ_M20.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M20.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16536966
Sequences flagged as poor quality0
Sequence length33
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC2754031.6653780385108126Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTAGGTATAGTAAATGTATTGAATCCATCATA212830.12869954500722805No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGG183120.11073373435006155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA295550.026.739671
CGTCTGA299000.026.1576916
ACGTCTG299950.026.04815715
CGGAAGA304800.025.8539414
ATCGGAA305700.025.760712
GCACACG306150.025.52969211
ACACGTC307000.025.45453513
CACACGT308650.025.3359512
CACGTCT312100.025.04708314
AGCACAC314950.024.93209310
TCGGAAG317700.024.7914123
GAACTCC317700.024.61818321
AGAGCAC321850.024.4384178
GAGCACA325050.024.2321519
AACTCCA325400.024.0697722
CAGTCAC317500.023.91560627
AAGAGCA331950.023.841247
ACTCCAG329200.023.72143223
TCCAGTC329300.023.62842825
GTCTGAA332850.023.56240717
Produced by FastQC (version 0.11.2)