FastQCFastQC Report
Sun 13 May 2018
LZ_M22.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M22.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19013128
Sequences flagged as poor quality0
Sequence length33
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC3369381.7721334437973593Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTAGGTATAGTAAATGTATTGAATCCATCATA247910.1303888555318199No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGG235820.12403009120855862No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTT218010.11466287924848557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA370850.026.7404121
ATCGGAA380200.026.027422
CGGAAGA391450.026.0098634
ACGTCTG389950.025.99152415
CGTCTGA390650.025.96554816
GCACACG396150.025.65603411
ACACGTC400850.025.3249713
CACACGT401450.025.2567912
AGCACAC406200.025.10434510
TCGGAAG395650.025.041573
CACGTCT408500.024.84079614
AGAGCAC412700.024.8100938
GAACTCC410500.024.74265921
AACTCCA417350.024.35253522
GAGCACA420100.024.3217839
CAGTCAC406400.024.07627
AAGAGCA428150.024.0661497
ACTCCAG423850.023.98544323
GTCTGAA424000.023.95810717
TCCAGTC427550.023.66761825
Produced by FastQC (version 0.11.2)