FastQCFastQC Report
Sun 13 May 2018
LZ_M23.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M23.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16908801
Sequences flagged as poor quality0
Sequence length33
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC4026602.381363409504908Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGGATAATAGTATGCCATTCCCCATTAATCTT390840.23114589851758266No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGG390680.23105127323930302No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATA315610.18665427548647595No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGA280820.16607919154054743No Hit
CCAGGATAATAGTATGCCATTCCCCATTAATCT267010.15791184720903628No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTC263590.15588923188580905No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGG236650.13995670065547522No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCC228820.13532597609966548No Hit
GCCAGGATAATAGTATGCCATTCCCCATTAATC226980.13423778539944967No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAAT226530.1339716518042882No Hit
CATTAATCTTTTCTACATTAAAGTTCCTTCCCG195350.11553155069954399No Hit
CTTTTCTACATTAAAGTTCCTTCCCGTAGAACT187830.11108416262040105No Hit
CCATTATCTTCTATCTTTTCTCTTTTGTCAGAG178050.10530019248555826No Hit
ATTATCTTCTATCTTTTCTCTTTTGTCAGAGGC178010.10527653616598835No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGA177800.10515234048824633No Hit
CAGAATATTCACCAGCCTTTTCTGTTTTGTCAG169710.10036784985523219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA430300.026.8184831
CGTCTGA433500.026.19377716
ACGTCTG433450.026.18472715
ATCGGAA440550.026.1394562
CGGAAGA440100.026.1378064
ACACGTC440450.025.79309713
GCACACG441800.025.76958511
TCGGAAG447650.025.7211693
CACACGT443500.025.63397812
AGCACAC449100.025.41984210
CACGTCT447300.025.39198914
GAACTCC449750.025.15454721
AGAGCAC455000.025.1398378
GAGCACA454850.025.1252029
AAGAGCA462750.024.8938247
AACTCCA454800.024.82558422
CAGTCAC447250.024.6889427
ACTCCAG459250.024.61105223
TCCAGTC458850.024.54726225
GAAGAGC477250.024.1883286
Produced by FastQC (version 0.11.2)