FastQCFastQC Report
Sun 13 May 2018
LZ_M25.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M25.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17268358
Sequences flagged as poor quality0
Sequence length33
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC2866321.6598682978427943Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA307250.026.693071
ACGTCTG310900.026.02948615
CGTCTGA311100.026.0034716
ATCGGAA317000.025.7792452
CGGAAGA317300.025.7117354
GCACACG321650.025.2437711
AGCACAC329450.024.70756510
ACACGTC330000.024.5763213
TCGGAAG333700.024.4724463
AGAGCAC333600.024.3586088
CACACGT333950.024.29775412
GAACTCC334900.024.2849121
CACGTCT337650.023.9953114
GTCTGAA339200.023.93684217
CAGTCAC331850.023.87354527
AACTCCA340950.023.81890522
GAGCACA342900.023.7581129
CTGAACT342950.023.67924519
TCCAGTC342300.023.46427725
AAGAGCA349900.023.3858977
Produced by FastQC (version 0.11.2)