FastQCFastQC Report
Sun 13 May 2018
LZ_M27.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M27.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21974639
Sequences flagged as poor quality0
Sequence length33
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC2143710.9755382102067752Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA232700.026.5589121
CGTCTGA248400.025.67759516
ACGTCTG249150.025.6545415
CGGAAGA253350.025.0848664
ATCGGAA247800.024.9504382
ACACGTC258400.024.72584213
GCACACG259300.024.650611
CACACGT262750.024.3370412
CACGTCT266850.023.9476114
AGCACAC271750.023.6349710
TCGGAAG266150.023.3463843
AGAGCAC277800.023.1784488
GAACTCC280500.022.87373521
GTCTGAA285350.022.51817517
AACTCCA286400.022.43541522
CAGTCAC279700.022.34060327
GAGCACA289400.022.2541529
AAGAGCA294000.022.0803347
ACTCCAG291600.022.01681523
CTGAACT292900.021.96498519
Produced by FastQC (version 0.11.2)