FastQCFastQC Report
Sun 13 May 2018
LZ_M28.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M28.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18287259
Sequences flagged as poor quality0
Sequence length33
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC3408101.863647252986355Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA366500.026.708381
CGTCTGA382850.026.17622216
ACGTCTG384750.026.11361715
ATCGGAA377100.025.9174772
CGGAAGA387850.025.9121424
GCACACG393750.025.55878411
ACACGTC393850.025.55544313
CACACGT397600.025.31441312
AGCACAC403150.025.01963210
CACGTCT402200.024.99064414
TCGGAAG394000.024.8363553
AGAGCAC408450.024.7412518
GAACTCC404750.024.59647221
GTCTGAA411150.024.36134317
AACTCCA410200.024.25644922
GAGCACA417400.024.2430829
CAGTCAC402750.024.19917127
CTGAACT416100.024.0455219
AAGAGCA421900.024.0165027
ACTCCAG414850.023.96829223
Produced by FastQC (version 0.11.2)