FastQCFastQC Report
Sun 13 May 2018
LZ_M29.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M29.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20537799
Sequences flagged as poor quality0
Sequence length33
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC5009792.439302283560181Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGTTTGAATATTTGCTACTACCACCAAGATC215350.10485544239672422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA540650.026.74311
CGTCTGA565050.026.34121116
ACGTCTG565850.026.30880415
CGGAAGA570850.026.08014
ACACGTC577900.025.79539513
CACACGT579900.025.7087612
GCACACG581000.025.70686511
TCGGAAG562150.025.6746183
ATCGGAA564000.025.6003482
AGCACAC585850.025.53054410
CACGTCT583750.025.5156414
GAACTCC586350.025.38632421
AGAGCAC594600.025.1797568
AACTCCA591150.025.15970222
CAGTCAC572800.025.05615627
GAGCACA601400.024.8793969
ACTCCAG597000.024.87924223
AAGAGCA607000.024.7965857
GTCTGAA605900.024.63434417
TCCAGTC603850.024.50983425
Produced by FastQC (version 0.11.2)