FastQCFastQC Report
Wed 9 May 2018
LZ_M2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20776078
Sequences flagged as poor quality0
Sequence length33
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC5294922.548565711006668Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTT439740.2116568873104924No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA409060.19688990385962163No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTT319730.1538933382903164No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTT309790.1491089896755297No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTT268610.12928811684284203No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATA258410.12437862430050561No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGA240290.11565705519588443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA572900.026.875771
CGTCTGA584950.026.43526316
ACGTCTG585200.026.40782715
CGGAAGA588700.026.3429644
ATCGGAA584750.026.2792952
ACACGTC593150.026.07435813
CACACGT592400.026.0185312
GCACACG594150.025.99867811
CACGTCT599250.025.79317514
AGCACAC602500.025.72123310
TCGGAAG598700.025.645743
AGAGCAC608150.025.4649228
GAACTCC614800.025.19173821
GAGCACA615350.025.175349
CAGTCAC603500.025.01170327
AACTCCA621150.024.9390922
GTCTGAA621100.024.90968517
ACTCCAG620100.024.88772223
AAGAGCA625950.024.8658477
TCCAGTC619550.024.83810425
Produced by FastQC (version 0.11.2)