FastQCFastQC Report
Sun 13 May 2018
LZ_M30.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameLZ_M30.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15087146
Sequences flagged as poor quality0
Sequence length33
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC3697642.4508545221210163Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA400100.026.7844141
ATCGGAA408450.026.1279832
CGTCTGA402550.026.09168416
ACGTCTG403550.026.07163615
CGGAAGA408000.026.0633874
ACACGTC412200.025.56701313
TCGGAAG418200.025.5085533
GCACACG413550.025.4837211
CACACGT413450.025.48318512
AGCACAC418400.025.2238110
CACGTCT417950.025.20558214
GAACTCC419050.024.96794121
AGAGCAC424500.024.9646348
AACTCCA423900.024.68962722
AAGAGCA432550.024.6779027
CAGTCAC417500.024.56329227
GAGCACA432500.024.4857569
ACTCCAG427750.024.45421423
GTCTGAA430700.024.40203517
CTGAACT432650.024.24221419
Produced by FastQC (version 0.11.2)